Zebrafish: a vertebrate tool for studying basal body biogenesis, structure, and function

Cilia

Table 1 Current zebrafish functional analysis, with zebrafish-specific references, of genes identified through the SCGS list to be BB/centrosome affiliated

Gene	RNA refseq/genbank	Associated disease	Genetic manipulation	Phenotype	Refs.
Ahi1	NM_001077561.1	JSRD	MO	CE, V, E, K, Ct, Hc, LRP, C−	[68]
Cep131	XM_009306856.1	BBS, T2D	MO	CE, E, LRP, BB+ , CLO	[15]
Bbs10	NM_001089463.1	BBS, T2D	MO	CE	[69, 70]
Bbs12	XM_002667206.3	ADPKD, NPHP, AS, OFDS, MKS, JS	MO	CE, LRP	[71]
Bbs5	NM_200299.1	BBS	MO	V, K, CLO	[72]
C2orf71	BI878361.1	RP	MO	V	[73]
Cep83	XM_009300427.1	Unknown	MO	E, CLO	[74]
Cep164	EB913016.1	NPHP	MO	CE, Mc	[75]
Cep290	NM_001168267.1	LCA, BBS, MKS, NPHP	MO	CE, V, K, Hc	[76, 77]
Cep41	NM_001002194.1	JSRD	MO	V, E, Hc, LRP, CM	[78]
Disc1	NM_001142263.1	SCZD	MO	V, Ct, Mc	[79]
Kif7	NM_001014816.1	ACLS, JS, HYLS	MO, ZFN Mt	Hh	[80, 81]
Mks1	NM_001077373.2	BBS, MKS	MO	CE	[82]
Nek2	NM_201050.1	RP	MO	V	[83]
Nin	XM_009307506.1	SS	MO	V, E, Mc	[84]
Ninl	NM_001281798.1	USH2A	MO	V, K, BB+	[85]
Odf2a	XM_001332528.6	MC	MO	V, Mc	[86]
Poc1b	NM_200118.1	CORD	MO	V, K, Ct, LRP, CLO	[53]
Rab11a	NM_001007359.1	Unknown	MO	LRP	[87]
Rp2	NM_213446.1	RP	MO	V, Hc, Mc	[88]
Rpgrip1 l	NM_001246660.2	JS, MKS, COACH	MO	CE, V, Hc, LRP, BB+, CLO	[89, 90]
Sass6	NM_213438.1	MC	MO, ENU Mt	MD	[48, 91]
Sdccag8	XM_005156579.2	BBS, SLS	MO	CE, K, Hc	[92]
Snx10a	NM_001139462.1	O	MO	LRP, C−	[93]
Stil	NM_173244.2	MC	MO, ENU Mt, RVI Mt.	V, Mc	[94, 95]
Toporsa	NM_001305555.1	RD	MO	CE, V, Hc	[96]
Ttk	NM_175042.2	Unknown	ENU Mt	CE	[97]
Yap1	NM_001139480.1	Unknown	MO	CE, V, K, Hc, BB+, C−, CLO	[29]
Gmnc	XM_009291838.1	Unknown	MO, CRISPR Mt	K, C−, CLO	[32]

Note the addition of post-SCGS genes, yap and gmnc
JSRD joubert syndrome and related disorders, BBS bardet biedl syndrome, T2D type 2 diabetes, ADPKD autosomal dominant polycystic kidney disease, NPHP nephronophthisis, AS alström syndrome, OFDS orofaciodigital syndrome type 1, MKS meckels syndrome, RP retinitis pigmentosa, LCA leber’s congenital amaurosis, MC microcephaly, USH2A usher syndrome 2A, COACH cerebellar vermis oligophrenia ataxia coloboma hepatic fibrosis, SCZD schizophrenia, SLS senior-loken syndrome, O osteopetrosis, CORD cone-rod dystrophy, RD retinal degeneration, ZFN zinc finger nuclease, ENU N-ethyl-N-nitrosourea, RVI retroviral insertion, Mt mutant. Phenotype abbreviations: CE convergent extension defects, V visual impairment, E ear and otolith defects, K kidney defects including pronephric tubule dilation, Ct abnormal cartilage development, Hc hydrocephaly, Mc microcephaly, LRP left–right patterning defects, BB+ BBs observed intact, C− cilia absent, CM defective cilia motility, CLO cilia length and organization affected, Hh hedgehog signaling abrogated, MD mitotic division disrupted

ISSN: 2046-2530