Fig. 3

Fibers attached to the basal bodies. The mature basal bodies and the probasal bodies are shown as white microtubules with the cartwheel at the proximal region shown in green. The two mature basal bodies are found at an oblique angle to one another and are about 400 nm in length. They are connected at their distal end by the distal striated fiber (red) and connected at the proximal end by the proximal striated fiber (purple). Lateral fibers (yellow) connect the mature basal body to its daughter probasal body via the VFL3 protein. Centrin (in red) is shown in the lumen of the basal body, in the stellate fiber, and in the nucleo-basal body connector (NBBC). The four-rootlet microtubules are shown with the four-membered (M4 and D4) and the two-membered (M2 and D2). One of the rootlet microtubules is associated with a striated fiber that contains SF-assemblin (orange). The transition fibers (blue) are shown on one of the basal bodies but each basal body has nine transition fibers. A microtubule is about 25 nm as an internal scale bar