Table 1 Known and candidate Giardia basal body proteins including supporting evidence

GL50803_6744

Centrin

Centrin

PF13499; PF13833

All basal bodies

HOM, IFA

GL50803_104685

Caltractin

Caltractin

PF13499

All basal bodies

HOM, IFA, GFP

GL50803_114218

Gamma-tubulin

Tubulin

PF00091; PF03953

All basal bodies, cytoplasm

HOM, PRO, IFA, GFP

GL50803_5462

Delta-tubulin

Tubulin

PF00091

Cytoplasm

HOM, GFP

GL50803_6336

Epsilon-tubulin

Tubulin

PF00091

Cytoplasm

HOM, GFP

GL50803_17429

GCP2

Spc97 Spc98

PF04130

n.d.

HOM

GL50803_12057

GCP3

Spc97_Spc98

PF04130

All basal bodies, median body, cytoplasmic caudal, posteriolateral, and anterior axonemes

HOM, GFP

GL50803_104150

Kinase, PLK

Ser/Thr kinase domain, POLO box domain

PF00069, PF00659

All basal bodies

EPI

GL50803_4689

Hypothetical protein

None

None

All basal bodies

PRO, EPI

GL50803_4692

Hypothetical protein

None

None

All basal bodies

PRO, EPI

GL50803_41512

Flagella associated protein Rib72

DUF1126 domain of unknown function

PF06565

All basal bodies, cytoplasm

PRO, EPI

GL50803_114546

Hypothetical protein

None

None

All basal bodies, all cytoplasmic axonemes

GFP

GL50803_3582

DUF390 domain containing protein

DUF390 domain of unknown function

PF04094

All basal bodies, all cytoplasmic axonemes

GFP

GL50803_8974

Hypothetical protein

None

None

All basal bodies, all cytoplasmic axonemes, median body, ciliary pocket

GFP

GL50803_16935

Hypothetical protein

None

None

All basal bodies, all flagella, ventral disc lateral crest, median body

GFP

GL50803_15218

WD-40 repeat protein

WD40 repeats

PF00400

All basal bodies, all flagella, ventral disc lateral crest, median body

PRO, IFA, GFP

GL50803_9117

CAMP-dependent protein kinase regulatory chain

Cyclic nucleotide binding domain

PF00027

All basal bodies, anterior axonemes, caudal axonemes

EPI

GL50803_11214

Kinase, AGC PKA

Kinase, AGC PKA

PF00069

All basal bodies, anterior axonemes, caudal axonemes

EPI

GL50803_16202

Axoneme central apparatus protein PF16/SPAG6

Central pair associated protein

PF00514

All basal bodies, anterior axonemes, posteriorlateral axonemes, caudal axonemes, median body

PRO, IFA, EPI

GL50803_11867

RIB43A

RIB43A

PF05914

All basal bodies, anterior axonemes, posteriorlateral axonemes, median body

EPI

GL50803_7351

Hypothetical protein

None

None

All basal bodies, ciliary pocket, cytoplasmic ventral, caudal, posteriolateral, and anterior axonemes

GFP

GL50803_24412

Ankyrin-repeat domain containing protein

Ankyrin-repeat domains

PF12796

All basal bodies, cytoplasmic anterior and posteriolateral axonemes

GFP

GL50803_10460

Hypothetical protein

None

None

All basal bodies, cytoplasmic anterior axonemes, median body

GFP

GL50803_17586

Ankyrin-repeat domain containing protein

Ankyrin-repeat domains

PF12796

All basal bodies, cytoplasmic anterior, posteriolateral and ventral axonemes

GFP

GL50803_7192

FWWh domain containing protein

FWWh domain of unknown function

PF14922

All basal bodies, cytoplasmic caudal axonemes, posteriolateral and anterior axonemes

GFP

GL50803_8557

Hypothetical protein

None

None

All basal bodies, cytoplasmic caudal axonemes, posteriolateral and anterior axonemes

GFP

GL50803_102455

GiKIN6a

Kinesin-6

PF00225

All basal bodies, cytoplasmic posteriolateral and anterior axonemes, median body, ventral disc

GFP

GL50803_11775

Kinase, ankyrin-repeat domain containing protein

Ser/Thr kinase domain, ankyrin-repeat domains

PF00069, PF1279

All basal bodies, cytoplasmic posteriolateral and caudal axonemes

GFP

GL50803_15219

Macoilin domain containing protein

Macoilin

PF09726

All basal bodies, cytoplasmic posteriolateral axonemes, cytoplasm

GFP

GL50803_15446

Hypothetical protein

None

None

All basal bodies, cytoplasmic posteriolateral axonemes, median body

GFP

GL50803_6709

Hypothetical protein

None

None

All basal bodies, median body, all flagella, ventral disc lateral crest

GFP

GL50803_92498

Kinase, NEK

Kinase, NEK

PF00069

All basal bodies, median body, anterior axonemes, posteriorlateral axonemes, caudal axonemes, ventral disc

EPI

GL50803_17154

RRP7 domain containing protein

Ribosomal RNA-processing protein 7 (RRP7) domain

PF12923

All basal bodies, median body, cytoplasmic anterior axonemes, caudal axonemes, posteriolateral axonemes

GFP

GL50803_4624

DUF4490 domain containing protein

DUF4490

PF14892

All basal bodies, median body, cytoplasmic caudal, ventral, posteriolateral and anterior axonemes

GFP

GL50803_13352

T-complex protein-10/Sas-4/CENPJ

T complex protein 10 family C terminal domain

PF07202

All basal bodies, median body, cytoplasmic posteriolateral axonemes, ciliary pocket

PRO, IFA, GFP

GL50803_10232

LRR repeat domain containing protein

CENP-F_leu_zip leucine-rich repeats

PF10473

All basal bodies, median body, ventral disc

GFP

GL50803_31671

RPB7 SHS2 domain containing protein

N terminal SHS2 domain of RNA pol II subunit RPB7

PF03876

All basal bodies, nuclei, cytoplasmic posteriolateral and anterior axonemes

GFP

GL50803_16220

Ankyrin-repeat domain containing protein

Ankyrin-repeat domains

PF12796

All basal bodies, plasma membrane, cytoplasm

GFP

GL50803_16973

Hypothetical protein

None

None

All basal bodies, posteriorlateral axonemes, median body

PRO, IFA

GL50803_17096

Ankyrin-repeat domain containing protein

Ankyrin-repeat domains

PF12796

All basal bodies, ventral disc lateral crest

GFP

GL50803_16424

MiflIP domain containing protein

Mlf1IP

PF10248

All basal bodies, ventral disc lateral crest, cytoplasmic anterior axonemes

GFP

GL50803_17097

Ankyrin-repeat domain containing protein

Ankyrin-repeat domains

PF12796

All basal bodies, ventral disc lateral crest, cytoplasmic anterior axonemes, median body

GFP

GL50803_101326

Hypothetical protein

None

None

All basal bodies, ventral disc lateral crest, cytoplasmic caudal axonemes

GFP

GL50803_15499

DUF390 domain containing protein

DUF390 domain of unknown function

PF04094

All basal bodies, ventral disc lateral crest, cytoplasmic posteriolateral axonemes

GFP

GL50803_5568

DUF866 domain containing protein

DUF866

PF05907

All basal bodies, ventral disc lateral crest, ventral axonemes

GFP

GL50803_5010

Ser/Thr phosphatase PP2A-2 catalytic subunit

Metallo-dependent phosphatases

PF00149

All basal bodies, ventral disc, anterior axonemes, posteriorlateral axonemes, caudal axonemes

PRO, IFA

GL50803_17563

Kinase, CMGC MAPK

Kinase, CMGC MAPK

PF00069

All basal bodies, ventral disc, caudal axonemes, median body

EPI

GL50803_16279

Kinase, NEK

Kinase, NEK

PF00069

All basal bodies, ventral disc, caudal axonemes, posteriorlateral axonemes

EPI

GL50803_5358

Aurora kinase

Aurora kinase

PF00069

All basal bodies, ventral disc, nuclei, anterior axonemes, median body

EPI

GL50803_15193

Hypothetical protein

None

None

Some basal bodies

PRO, IFA, GFP

GL50803_29796

Hypothetical protein

None

None

Some basal bodies, anterior flagella, ventral flagella, median body

GFP

GL50803_6254

Hypothetical protein

None

None

Some basal bodies, cytoplasmic posteriolateral axonomes

GFP

GL50803_15455

PACRG1

Parkin coregulated protein

PF10274

Cytoplasm, nuclei

HOM, GFP

GL50803_14048

EB1

EBl-like C terminal motif

PF03271

Nuclear membrane, median body

GFP

GL50803_15956

FAP52

WD40 repeats

PF00400

Plasma membrane, all axonemes

HOM, GFP

GL50803_5333

Calmodulin

EF-hand domain

PF13499

n.d.

HOM

GL50803_33762

POC1

WD repeat protein

PF00400

n.d.

HOM

GL50803_32375

POC18

None

None

n.d.

HOM

GL50803_13372

FAP45

TPH domain

PF13868

n.d.

HOM

GL50803_5167

VFL3

None

None

n.d.

HOM

GL50803_15248

Bub2

TBC domain

PF00566

n.d.

HOM

GL50803_8738

BBS1

BBS1

PF14779

n.d.

HOM

GL50803_23934

BBS2

None

None

n.d.

HOM

GL50803_10529

BBS4

TPR_1 Tetratricopeptide repeat

PF01515

n.d.

HOM

GL50803_8146

BBS5

DUF1448 domain of unknown function

PF07289

n.d.

HOM

GL50803_8508

BBS8

TPR_1 Tetratricopeptide repeat

PF01515

n.d.

HOM