Fig. 8
From: Fixation methods can differentially affect ciliary protein immunolabeling
Different fixation methods affect IFT20 labeling of cis-Golgi in IMCD3 and RPE cells. IMCD3 and RPE cells were subjected to various fixation methods and then co-stained with IFT20 (green) and GM130 (a cis-Golgi marker; red). All coverslips were also stained with Hoechst for nuclear labeling (blue). Co-labeling was only observed in the methanol and CB-PFA groups. Scale bars 10 µm