Fig. 9
From: Fixation methods can differentially affect ciliary protein immunolabeling
Summary of general fixation guidelines. PFA fixation preserved most membrane-localized cilia proteins and tubulin post-translational modification immunolabeling. Application of a cytoskeletal buffer (CB) wash or pre-fixation permeabilization with Triton X-100 (not shown) washed away many membrane-cilia proteins, but revealed axonemal-cilia and post-translational modification immunolabeling. Fixation in PFA prepared in CB (CB-PFA) almost always preserved membrane-cilia proteins, axonemal-cilia proteins, and post-translational modification of tubulin immunolabeling