Fig. 3

LARP6 is necessary for MCC differentiation. a Absence of cilia on epidermal MCC of LARP6 morphants. L6MO (15 ng/blastomere) with memRFP mRNA or conMO with memGFP mRNA were injected into the right side or the left side, respectively. Embryos were fixed at stage 25. Cilia were stained with an anti-acetylated tubulin antibody. Following staining, lateral explants were prepared from embryos and images from the right (L6MO) or left (conMO) lateral side were taken by confocal microscopy. b Rescue experiments with hL6 or Δ1-478 mRNA in LARP6 morphant MCCs. c–e B9D1, γ-tubulin and centrin proteins were not detected at the LARP6 morphant epidermis. Axonemes and ciliary transition zones or basal bodies were stained by an anti-acetylated tubulin and anti-B9D1, anti-γ-tubulin or anti-centrin antibody, respectively. Following staining, lateral explants were prepared from embryos and images were taken by confocal microscopy. Arrowheads indicate examples of centrioles. f The depletion of LARP6 did not change total amounts of γ-tubulin and centrin proteins in embryos. g The localization of LARP6 in MCCs. N nucleus. An Arrowhead indicates an example of a cilium. Scale bars 20 µm in (a), 10 µm in (b–e, g). n indicates number of embryos examined