Table 2 Characteristics and potentials of three airway epithelial culture methods
Crude immediate brush material [21] | ||||
|---|---|---|---|---|
Characteristics | ||||
Sampling of cells | Nasal brush biopsy | Nasal brush biopsy | Removal of nasal polyp or nasal forceps biopsy | Removal of nasal polyp/nasal brush biopsy |
Anesthesia required | No | No | Yes | Yes (polyp removal) No (nasal brush) |
Preparation steps | Cell collection Cell dispersion | Cell collection Cell dispersion Cell proliferation Ciliary regeneration | Tissue washing Enzymatic tissue digestion Cell dispersion Cell proliferation Ciliary regeneration | Cell collection Cell dispersion Spheroid formation |
Longevity of cells/culture | 1–3 days | >1 month | >1 month | At least 16 days |
Time from sampling to analysis | Immediately | 4 weeks | 4–6 weeks | 24 h |
Materials and reagents | Falcon tube NaCl Glass slide | Falcon tube Culture medium T25/75 flask 24-well plate Transwell insert filters D-PBS Trypsin–EDTA Trypsin-inhibitor Centrifuge CO2 incubator | Falcon tube Pronase Culture medium Plastic plate Collagen gel Collagenase T25/75 flask Gyrotori shaker CO2 incubator | Falcon tube Culture medium 24-well plate CO2 incubator |
Complexity | Simple | Complex | Complex | Simple |
Terminally differentiated cells | Yes | No | No | Yes |
Cell divisions | No | Yes | Yes | No |
Ciliogenesis | No | Yes | Yes | No |
Known applications | ||||
Diagnostics | Yes | Yes | Yes | Yes |
Study of transport mechanisms | No | Yes | No | Yes |
Study of ciliary physiology | Yes | Yes | Yes | Yes |
Potential applications | ||||
Study of drug effects | No | Yes | Yes | Yes |