Fig. 1
From: Amyloid-β interrupts canonical Sonic hedgehog signaling by distorting primary cilia structure
Synthetic Amyloid-β decreases cilia length. a Confocal immunofluorescence analysis of naïve NIH3T3 cells treated with synthetic Aβ42 peptide for 24 h. Cells were stained with acetylated tubulin and > 50 cilia per condition analyzed. Scale bar represents 20 μm. b Quantification of naïve NIH3T3 cells exposed to synthetic human Aβ42 for 24 h. Cilia length was analyzed using an anti-acetylated tubulin antibody and immunofluorescence, 30–50 cells were analyzed using Volocity 3D analysis software. Values denote mean ± standard errors of the means. Student’s t test was used for statistical analysis: ***p < 0.005, **p < 0.01, *p < 0.05