Fig. 2

Arl13b:GFP-expressing glioma cell lines were generated using PiggyBac transgenesis. a PiggyBac transgenesis was used to insert GFP-tagged, full-length mouse Arl13b into the genome of GBM patient-derived cells. Three to four weeks after transfection, cells were FAC-sorted and GFP⁺ clones expanded and analyzed for the presence of GFP⁺ cilia. b Quantification of the percentage of acetylated alpha-tubulin⁺ (aaTub⁺) cilia that were Arl13b:GFP⁺ across seven Arl13b:GFP⁺ L0 clones. c Confocal maximum projection image of an unstained L0 clone C6 tumor sphere expressing Arl13b:GFP⁺ cilia (green). Nuclei were labeled with DAPI (blue). Scale bar = 10 μm