Fig. 9

Rootlet angles relatively constant in the control and SFA protein-depleted cells. a The enlarged image of an individual basal body with all three rootlets [TR (green), PR (green), and SR (red)] along with basal bodies (green). b The method showing how the angles were measured: First a circle was drawn around the basal body; then three straight lines were drawn to determine the positions of rootlets to the basal body; finally, angles were measured by protractor. c, d The measurement of angles between PRs and TRs (different group: Mean ± SEM (standard error of the mean); Control: 108 ± 0.56; Paralog Group 2: 107.7 ± 0.44; Structural Group 1: 107.1 ± 0.43; Structural Group 107.2 ± 0.50) and between PRs and SRs (different group: Mean ± SEM; Control: 134.9 ± 0.68; Paralog Group 2: 135.9 ± 0.69; Structural Group 1: 136.7 ± 0.83; Structural Group 136.4 ± 0.77) in control, Paralog Group 2-, S-tructural Group 1-. and Structural Group 2-depleted cells, respectively. Angle values (both for PRs vs TRs and PRs vs SRs) from different groups are not statistically significant (P value < 0.5; unpaired t-test) compared to control cell