Figure 9

Loss of Bmp2 and Bmp4 expression in cbs mutant OFT and endocardial cushions. (A,B) In situ hybridization of Bmp2 (A,C) and Bmp4 (B,D) in the OFT (arrow) of E10.5 wild type (+/+) and cbs/cbs hearts. For each transverse section, dorsal is to the right. ‘SM’: splanchnic mesoderm. (C,D) In situ hybridization analysis of E12.5 wild type (+/+) and cbs/cbs embryonic hearts. For each transverse section, dorsal is to the top. ‘V’: right ventricle, EC: endocardial cushion. Arrowheads indicate signals described in text. Scale bars: A,B, 200 μm; C,D, 100 μm. cbs, cobblestone; E10.5,embryonic day 10.5; OFT, outflow tract.