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The mitotic spindle protein SPAG5/Astrin connects to the Usher protein network postmitotically
Cilia volume 1, Article number: 2 (2012)
Mutations in the gene for Usher syndrome 2A (USH2A) are causative for non-syndromic retinitis pigmentosa and Usher syndrome, a condition that is the most common cause of combined deaf-blindness. To gain insight into the molecular pathology underlying USH2A-associated retinal degeneration, we aimed to identify interacting proteins of USH2A isoform B (USH2AisoB) in the retina.
We identified the centrosomal and microtubule-associated protein sperm-associated antigen (SPAG)5 in the retina. SPAG5 was also found to interact with another previously described USH2AisoB interaction partner: the centrosomal ninein-like protein NINLisoB. Using In situ hybridization, we found that Spag5 was widely expressed during murine embryonic development, with prominent signals in the eye, cochlea, brain, kidney and liver. SPAG5 expression in adult human tissues was detected by quantitative PCR, which identified expression in the retina, brain, intestine, kidney and testis. In the retina, Spag5, Ush2aisoB and NinlisoB were present at several subcellular structures of photoreceptor cells, and colocalized at the basal bodies.
Based on these results and on the suggested roles for USH proteins in vesicle transport and providing structural support to both the inner ear and the retina, we hypothesize that SPAG5, USH2AisoB and NINLisoB may function together in microtubule-based cytoplasmic trafficking of proteins that are essential for cilium formation, maintenance and/or function.
Mutations in the gene for Usher syndrome 2A (USH2A) are causative for non-syndromic recessive retinitis pigmentosa (RP) [1–4] and for Usher syndrome type II (USH2), a recessive disease characterized by congenital moderate to severe stable hearing loss, and RP that often leads to blindness . Mutations in this gene probably account for 8 to 20% of the autosomal recessive RP cases [3, 6], and are suggested to be the commonest cause of RP in the USA . It is estimated that up to 85% of patients with USH2 and about half of all patients with Usher syndrome have mutations in USH2A . All proteins encoded by genes associated with USH1 and USH2 are present in hair cells and photoreceptor cells, and are interconnected in a network of interacting proteins [8–12].
To gain insight into the molecular pathology of retinal degeneration resulting from USH2A mutations, we aimed to determine the retinal repertoire of USH2AisoB-interacting proteins. By using the intracellular domain of USH2AisoB as bait in an interaction trap screen of a retinal cDNA library expressed in yeast (yeast two-hybrid screening), we recently identified the centrosomal protein NINLisoB, previously known as Nlp (ninein-like protein). NinlisoB colocalized with Ush2aisoB at centrioles, basal bodies and in the periciliary regions of photoreceptor cells . We hypothesized that NINLisoB functions in handing over cargo vesicles from the transport system of the inner segment to the intraflagellar transport (IFT) machinery that is involved in transport through the connecting cilium [13, 14]. Thereby, NINLisoB may function in the development and maintenance of the connecting cilium and outer segment .
In addition to NINLisoB, another centrosomal and microtubule-associated protein was identified in the yeast two-hybrid screen, namely sperm-associated antigen (SPAG)5, also called astrin. SPAG5 was originally identified as a microtubule-associated protein with dual localization to both centrosomes and kinetochores, and is required for mitotic spindle formation and chromosome segregation [15, 16]. Targeting of SPAG5 to the centrosome during the S and G2 phases of the cell cycle is mediated by ninein, and the SPAG5-ninein interaction is required for the maintenance of centrosome/spindle pole integrity . Interestingly, ninein is a paralog of NINL, which prompted us to investigate the interaction between SPAG5 and NINLisoB. In this study, we describe the specific interaction between SPAG5 and both USH2AisoB and NINLisoB, and their (partial) colocalization in photoreceptor cells. Our results suggest that these proteins function directly or indirectly in the microtubule-based vesicle transport that is essential for the long-term maintenance and/or function of photoreceptor cells.
Interaction of SPAG5 with USH2AisoB and NINLisoB
A yeast two-hybrid (Y2H) screen of an oligo-d(T) primed human retinal cDNA library was performed to identify interaction partners of USH2AisoB, by using its intracellular domain (ICD; USH2AisoBICD) as a bait. From a group of clones that activated all four reporter genes, two identical clones, encoding SPAG5 amino acids (aa) 774 to 1193, were identified (Figure 1A). The interaction between USH2AisoB and SPAG5 was confirmed by a glutathione S-transferase (GST) pull-down assay, in which full-length Flag-tagged SPAG5 was efficiently pulled down from COS-1 cell lysates by GST-fused USH2AisoBICD but not by GST alone (Figure 1B). To determine the interacting epitopes of the proteins, we performed an Y2H analysis using constructs encoding fragments of USH2AisoBICD (aa 5064 to 5202) and the SPAG5 Y2H clone. The USH2AisoB peptide containing aa 5064 to 5196 was determined to interact with the SPAG5 fragment containing aa 973 to 1193 (Figure 1A). To further validate the interaction, we performed a co-immunoprecipitation assay from COS-1 cells. This assay showed that hemagglutinin (HA)-tagged SPAG5 aa 973 to 1193 specifically co-immunoprecipitated with the GFP-tagged USH2AisoBICD peptide (Figure 1C).
In the described Y2H screen with the USH2AisoBICD peptide as bait, NINLisoB was also identified as an interaction partner . Interestingly, Cheng and co-workers previously found that SPAG5 also interacts with ninein , which is a paralog of NINL. We therefore assessed the interaction between SPAG5 and NINL also. In a liquid β-galactosidase assay, both full-length NINLisoB and the predicted NINLisoB intermediate filament (IF) domain (aa 656 to 925) were found to interact with SPAG5 aa 973 to 1193 (Figure 2A). The interaction strength between SPAG5 aa 973 to 1193 and full-length NINLisoA was significantly weaker (data not shown). The interaction between SPAG5 and NINLisoB was confirmed by a GST pull-down assay and by co-immunoprecipitation. Flag-tagged full-length NINLisoB was efficiently pulled down from COS-1 lysates by GST-fused SPAG5 aa: 973 to 1193 (Figure 2B), and it also co-immunoprecipitated with HA-tagged full-length SPAG5 (Figure 2C). From these experiments, we concluded that SPAG5 interacts with both USH2AisoB and NINLisoB.
SPAG5 expression in human adult tissues and during murine development
SPAG5 is known to play a role in cell proliferation, and therefore an important function during development could be expected. To examine this, we determined the expression pattern of SPAG5 in human adult tissues by quantitative (q)PCR, using primers in exons 12 and 13. Of the examined human adult tissues, expression of SPAG5 was mainly detected in the retina, brain, duodenum, kidney and testis (Figure 3A).
We used RNA in situ hybridization (ISH) to determine the expression of Spag5 during development. ISH was performed using mouse embryos at embryonic day (E)12.5 to 18.5, and mouse eyes at postnatal day (P)7. Spag5 was found to be widely transcribed during murine embryonic development. At E12.5 and E14.5, transcripts were detected in the eye, cochlea, forebrain, midbrain, kidney, liver, lung, adrenal gland, thymus, submandibular (salivary) glands, primordial vibrassal follicles, limbs, upper and lower jaws, intestine, heart, olfactory epithelium, and testes (Figure 3B-C, 3E-G; other data not shown). From E16.5 onwards, Spag5 transcripts were mainly present in the eye, brain, kidney, lung, thymus, liver, and primordial vibrassal follicles (Figure 3D, H-K; other data not shown). From E12.5 to E18.5, Spag5 transcripts were seen in the neuroblastic layer of the retina (Figure 3F-K), and at E16.5 (Figure 3I) and E18.5 (Figure 3K) these transcripts were detected more specifically at the (developing) inner nuclear layer (INL) and in low amounts in the ganglion cell layer (GCL). This pattern was maintained at P7 (Figure 3L-M). Hybridizations with a sense cRNA probe did not detect any signal, confirming the specificity of the assay (data not shown).
Spag5 localization in the retina
Because SPAG5 was identified from a retinal cDNA library, and Spag5 mRNA was expressed in the developing and the adult retina, we set out to determine its retinal subcellular localization. For this purpose, rat cryosections (P20) were co-stained with affinity-purified anti-SPAG5 antibodies (green; Figure 4A-Aii) and monoclonal anti-pan-centrin antibodies (20H5, red; Figure 4B-Bii) as markers for the basal body, connecting cilium, accessory centriole of photoreceptor cells, and centrioles in both the INL and the GCL . Spag5 was predominantly detected at the basal body of photoreceptor cells, and at one of the two centrosomal centrioles in both the INL and GCL, as shown by partial colocalization with centrins that mark these structures (yellow; Figures 4C-Cii; other data not shown). In addition, Spag5 immunostaining was weakly detected at the distal part of the connecting cilium (CC; Figure 4A-Ai open arrowhead), in the accessory centriole, at the outer limiting membrane (OLM; Figure 4A-Ai, filled arrowhead) and at the outer plexiform layer (OPL) (Figure 4A-Ai).
To determine the Spag5 distribution in the photoreceptor cells in more detail, we used immunoelectron microscopy. Spag5 was detected around the basal body (BB; Figure 5A-B) and at the collar-like extension (CE) of the apical inner segment (Figure 5A-C). In some photoreceptors, labeling was detected in the region of the rootlet (R; Figure 5A). In previous studies, immunoelectron microscopy showed subcellular localization of Ush2aisoB and NinlisoB at these structures as well, although both proteins were detected exactly at the basal body [10, 13], whereas Spag5 was mainly located around it.
Spag5 colocalization with Ush2aisoB and NinlisoB in photoreceptor cells
To investigate whether Spag5 and Ush2aisoB colocalize in the retina, we performed immunostaining on retinal cryosections using affinity-purified antibodies against SPAG5 (red; Figure 6A) and USH2AisoB (green; Figure 6B), which revealed partial colocalization of these proteins at the basal body (yellow; Figure 6C). The same analysis was performed for SPAG5 and NINLisoB. Using affinity-purified antibodies against SPAG5 (green; Figure 6D) and NINLisoB (red; Figure 6E), we detected partial colocalization at the basal body/centriole in the region of the connecting cilium and in the INL (yellow; Figure 6F and data not shown). Although staining was weak for anti-SPAG5, both antibodies also stained the ciliary rootlets (Figure 6Di-Ei). The rootlet localization of SPAG5 was confirmed indirectly by co-immunostaining (yellow; Figure 6I) using antibodies against NINLisoB (green; Figure 6G) and rootletin (red; Figure 6H).
In this study, we identified SPAG5 as a novel member of the Usher protein network via its interaction with both USH2AisoB and NINLisoB. SPAG5 is a non-motor microtubule-associated protein that is essential for progression through mitosis [15, 16, 19, 20]. We found that Spag5 is widely expressed during mouse development, which is consistent with its function in cell proliferation. However, we also detected Spag5 in photoreceptor cells, indicating that Spag5 also plays a postmitotic role in differentiated ciliated cells.
During mitosis, SPAG5 localizes to centrosomes and to spindle and kinetochore microtubules, and is required for correct mitotic spindle formation, sister chromatid cohesion and centrosome integrity [15, 16, 19–21]. By contrast, the interaction partner of SPAG5, NINLisoB, mainly fulfills a role during interphase, in which it is involved in microtubule organization and nucleation [22–24]. However, during cytokinesis at the end-stage of mitosis, SPAG5 and NINL are detected at the midzone microtubules [15, 21] and the midbody [13, 25], respectively. Depletion of SPAG5 results in cellular growth arrest with disorganized multipolar spindles, which ultimately leads to (p53-dependent) apoptosis [16, 20, 26]. NINL is required for proper centrosome maturation and spindle assembly, and NINL abnormalities may contribute to genomic instability and tumorigenesis [22, 27, 28]. Hence, both SPAG5 and NINL are essential for proper cell proliferation.
After exit from the cell cycle, a primary cilium develops, which essentially is a microtubule-based antenna-like structure that protrudes from the (apical) surface of many cell types. Cilia are involved in a wide variety of sensory functions, such as chemical, mechanical and photo sensation, Hedgehog signaling, and control of cell growth [29, 30]. Cilia assembly and disassembly are closely coupled to the cell cycle . Therefore, it is not surprising that similar protein complexes play a role in both cell-cycle regulation and cilia assembly and function. Indeed, Smith and co-workers recently found that the proteomes of the midbody and of the cilia/basal body significantly overlap . Furthermore, they reported the presence of a number of central spindle/midbody proteins, which control cytokinesis during mitosis, at the basal body/rootlet of ciliated cells, where they were suggested to control cilia morphogenesis and function .
The outer segment of the postmitotic photoreceptor cell is a highly specialized sensory cilium, with the connecting cilium as the ciliary transition zone [33, 34]. Both the proteins involved in the formation and maintenance of the outer segments and the proteins involved in the visual cycle are synthesized in the inner segment, and actively transported to the outer segment via involvement of the IFT machinery [14, 35]. A significant part of protein transport towards the basal body is via trans-Golgi derived vesicles that migrate along microtubules. These vesicles are thought to dock in the region of the basal body and at the membrane of the apical inner segment that surrounds the connecting cilium as a collar [10, 14, 36, 37]. At the central spindle and midbody, a protein network, including proteins that are also present at the basal body, organizes the microtubule bundles and vesicle recruitment for cell abscission . Based on this and on the (co)localization of Spag5 and NinlisoB at the basal body, the rootlet and the collar-like apical inner segment of photoreceptor cells (Figure 5, Figure 6) , we propose that both proteins function in the development and/or function of the photoreceptor cilium by direct involvement in vesicle transport towards the basal body and possibly the rootlet, or by an indirect role in microtubule organization. More specifically, NinlisoB might function as an adapter protein in this retrograde transport through its association with the dynactin p50-dynamitin and p150Glued subunits of the dynein-dynactin motor complex [13, 23]. This complex also transports kinetochore-components from kinetochores to spindle poles during mitosis . As Spag5 is present at kinetochores and spindle poles during mitosis [15, 16], it may serve as cargo of this complex. Therefore, Spag5 may be transported towards the basal body by the dynein-dynactin motor complex via its interaction with NinlisoB, analogous to the targeting of Spag5 to the centrosome by the Ninl paralog, ninein, during the cell cycle . At the basal body, Spag5 may have a structural and organizational role by bundling microtubules or crosslinking them to other components, similar to its role during mitosis [15, 16]. Because both SPAG5 and NINLisoB associate with the cytoplasmic region of USH2AisoB, these proteins may well be involved in microtubule-based transport of USH2AisoB-containing vesicles towards the membrane of the apical inner segment of photoreceptor cells. Indeed, Zallocchi and co-workers provided evidence for the existence of USH protein complexes in vesicles of tracheal epithelium, and their results implied that the ectodomain of Ush2aisoB faces the lumen of vesicles, with its cytoplasmic domain towards the cytosol .
The association of SPAG5 with both USH2AisoB and NINLisoB and its localization in photoreceptor cells pinpoints SPAG5 as a functional candidate gene for retinal ciliopathies. This hypothesis is supported by the observed expression of Spag5 in tissues such as retina, kidney, brain and testis, which are often affected in ciliopathies.
Two Spag5 animal models have been reported. Spag5-null mice were found to be viable and fertile, and did not display a clear phenotype . However, defects may have been missed, as this model is probably phenotyped with a focus on spermatogenesis and fertility anomalies. The rat model is characterized by male sterility, reduced female fertility, hypogonadism, progressive renal insufficiency and body growth retardation, as a consequence of hypogenesis that might result from defective cell proliferation [41, 42]. The renal phenotype is similar to oligomeganephronia [43, 44], the most common form of human congenital renal hypoplasia, which in a few cases is found to be associated with RP, hearing impairment and stunted growth . SPAG5 is a candidate gene for association with the clinical features in the patient described by Janin-Mercier and co-workers . No retinal dysfunction has been reported in the Spag5 animal models, thus a detailed retinal examination is needed to exclude defects in visual function; unfortunately, the models were no longer available for this analysis.
In conclusion, our studies show that SPAG5 associates with both USH2AisoB and NINLisoB. Therefore, it seems likely that besides its known function in cell proliferation, SPAG5 has a role in differentiated cells such as photoreceptor cells. Our data suggest that SPAG5 and NINLisoB function directly or indirectly in the microtubule-based transport and docking of (USH2AisoB-containing) vesicles to the apical inner segment and basal body of photoreceptor cells, which is essential for their long-term maintenance and/or function. In continuation with its expression pattern, this indicates that SPAG5 is a very likely candidate gene for (retinal) ciliopathies.
The Wistar rats and C57BL6 JOlaHsD mice (Harlan, Horst, The Netherlands) used in this study were housed in standard cages and received water and food ad libitum. All experiments were conducted according to international and institutional guidelines.
Plasmids and antibodies
The human image clone IRAUp969C082D (ImaGenes.GmbH, Berlin, Germany) was used as a template to amplify full-length SPAG5. The amino acids were numbered according to the entries in GenBank (NP_006452 (SPAG5), NP_996816 (USH2A), EU718622) (NINLisoB; https://www.ncbi.nlm.nih.gov/protein; provided in the public domain by the National Center for Biotechnology Information, Bethesda, MD) All constructs were generated using commercial cloning technology (Gateway; Invitrogen, Carlsbad, CA, USA), according to the manufacturer's instructions.
Antibodies against the C-terminal region of SPAG5 were used, which were raised in guinea pigs against a GST-fusion protein encoding a peptide consisting of aa 1063 to 1187. The cDNA encoding this peptide was amplified by using the forward and reverse primers 5'-GGCGAGCTCATAAGCCTTAG-3' and 5'-TCCCTGTAGTTCTTTGC-3', respectively. The affinity-purified SPAG5 antibodies did not stain tissue immunohistochemically and they did not detect SPAG5 on western blot after pre-adsorption of the primary antibodies with the antigen (Figure 7). Antibodies against rootletin (Novus Biologicals, Cambridge, UK) were used at 1:1000 dilution. The monoclonal antibodies directed against centrins, and the polyclonal antibodies directed against NINL and the cytoplasmic region of USH2AisoB have been described previously [12, 13, 46]; all were used at 1:250 dilution. Anti-HA and anti-Flag (Sigma-Aldrich, Munich, Germany) were used at 1:1000 dilution. The secondary antibodies were goat anti-guinea pig Alexa Fluor 488 and Alexa Fluor 568, goat anti-rabbit Alexa Fluor 568, goat-anti-human Alexa Fluor 568, IRDye800 goat anti-guinea pig IgG, IRDye800 goat-anti-mouse, and IRDye800 goat anti-rabbit (all used at 1:500 dilution, and all from Molecular Probes-Invitrogen Carlsbad, CA, USA).
Yeast two-hybrid analysis
A GAL4-based yeast two-hybrid system (HybriZAP, Stratagene, La Jolla, CA, USA) to identify proteins that interact with the cytoplasmic region of USH2AisoB (aa 5064 to 5202; NP_996816) was used as previously described [13, 47].
The GST-fusion proteins were produced by transforming Escherichia coli BL21-DE3 with plasmids pDEST15-USH2A_ICD (aa 5064 to 5202) or pDEST15-SPAG5 (aa 973 to 1193) respectively, as previously described . Flag-tagged SPAG5 or Flag-tagged NINLisoB were produced by transfecting COS-1 cells with plasmids encoding p3xFlag-SPAG5 or NINLisoB, respectively, using a transfection reagent (Effectene; Qiagen, Hildene, Germany) according the manufacturer's instructions. The GST pull-down assay was performed as described previously .
Immunohistochemistry in rat retina
Unfixed eyes removed from 20-day-old (P20) Wistar rats were isolated and frozen in melting isopentane. Cryosections 7 μm thick were treated and immunolabeled as described previously . Immunofluorescence was visualized under a fluorescent microscope (Axio Imager Z1; Zeiss, Basle, Switzerland) equipped with a camera (AxioCam MRm; Zeiss). Images were processed using Axiovision Rel (version 4.6) and Adobe Photoshop (Adobe Systems, San Jose, CA, USA).
Expression by real-time quantitative PCR
Total RNA was used from several human adult tissues (Agilent Technologies, Santa Clara, CA, USA) and cDNA was generated using reverse transcriptase according the manufacturer's instructions (iScript; Bio-rad laboratories, Hercules, CA, USA). cDNA primers were designed using Primer3 and validated for SPAG5 and the reference gene glucuronidase-β (GUSB) (primers: SPAG5 exon 12 to 13 forward 5'-ACTCTGCCAGCTTACCCAG-3' and SPAG5 reverse 5'-CAGTTCTGCCTGCATGTG-3', GUSB forward 5'-AGAGTGGTGCTGAGGATTGG-3' and GUSB reverse 5'-CCCTCATGCTCTAGCGTGTC-3). Quantitative (q)PCR was performed using adult human cDNA as a template and the SYBR Green PCR master mix (Applied Biosystems, Life Technologies, Foster City, CA, USA). PCR program: at 95°C for 10 minutes, 40 cycles at 95°C for 15 seconds and 60°C for 30 seconds, followed by 60 cycles at 95°C for 1 minute, 65°C for 1 minute (7500 Fast Real-Time PCR System; Applied Biosystems). Data were analyzed using the system software SDS (version 220.127.116.11).
Mouse RNA probes in situ hybridization
A probe corresponding to nucleotides 2698 to 3757 [GenBank: NM_017407], which recognizes mouse Spag5 transcripts was generated from mouse retina cDNA (Marathon; Clontech, USA) with the forward and reverse primers 5'-ATACCTGTGCAGGCTGGAG-3' and 5'-GTTCTCTTCCAAGTCGTGTC-3', respectively. Digoxigenin labeling was performed as previously described . C57Bl6 Jlco mouse embryos were collected at various embryonic stages (E12.5 to E18.5), and mouse eyes at P7. RNA in situ hybridization was performed as previously described .
Pre-embedding immunoelectron microscopy
Vibratome-cut sections of mouse retina were incubated with a an antibody (1 100 dilution) against SPAG5 and visualized by a secondary antibody (Vectastain ABC Kit, Vector Laboratories, Peterborough, UK). After fixation with 0.5% OsO4, the specimens were embedded in araldite, and ultrathin sections were viewed with a transmission electron microscope (Tecnai 12; FEI, Hillsboro, OR), as described previously .
ganglion cell layer
inner nuclear layer
ninein-like protein isoform B
Usher syndrome type II, : yeast two-hybrid.
Kaiserman N, Obolensky A, Banin E, Sharon D: Novel USH2A mutations in Israeli patients with retinitis pigmentosa and Usher syndrome type 2. Arch Ophthalmol. 2007, 125: 219-224. 10.1001/archopht.125.2.219.
Liu X, Tang Z, Li C, Yang K, Gan G, Zhang Z, Liu J, Jiang F, Wang Q, Liu M: Novel USH2A compound heterozygous mutations cause RP/USH2 in a Chinese family. Mol Vis. 2010, 16: 454-461.
McGee TL, Seyedahmadi BJ, Sweeney MO, Dryja TP, Berson EL: Novel mutations in the long isoform of the USH2A gene in patients with Usher syndrome type II or non-syndromic retinitis pigmentosa. J Med Genet. 2010, 47: 499-506. 10.1136/jmg.2009.075143.
Rivolta C, Sweklo EA, Berson EL, Dryja TP: Missense mutation in the USH2A gene: association with recessive retinitis pigmentosa without hearing loss. Am J Hum Genet. 2000, 66: 1975-1978. 10.1086/302926.
Smith RJ, Berlin CI, Hejtmancik JF, Keats BJ, Kimberling WJ, Lewis RA, Moller CG, Pelias MZ, Tranebjaerg L: Clinical diagnosis of the Usher syndromes. Usher Syndrome Consortium. Am J Med Genet. 1994, 50: 32-38. 10.1002/ajmg.1320500107.
Hartong DT, Berson EL, Dryja TP: Retinitis pigmentosa. Lancet. 2006, 368: 1795-1809. 10.1016/S0140-6736(06)69740-7.
Cohen M, Bitner-Glindzicz M, Luxon L: The changing face of Usher syndrome: clinical implications. Int J Audiol. 2007, 46: 82-93. 10.1080/14992020600975279.
Adato A, Michel V, Kikkawa Y, Reiners J, Alagramam KN, Weil D, Yonekawa H, Wolfrum U, El Amraoui A, Petit C: Interactions in the network of Usher syndrome type 1 proteins. Hum Mol Genet. 2005, 14: 347-356.
Kremer H, van Wijk E, Marker T, Wolfrum U, Roepman R: Usher syndrome: molecular links of pathogenesis, proteins and pathways. Hum Mol Genet. 2006, 15 (Spec No 2): R262-R270.
Maerker T, van Wijk E, Overlack N, Kersten FFJ, McGee J, Goldmann T, Sehn E, Roepman R, Walsh EJ, Kremer H, Wolfrum U: A novel Usher protein network at the periciliary reloading point between molecular transport machineries in vertebrate photoreceptor cells. Hum Mol Genet. 2008, 17: 71-86.
Reiners J, van Wijk E, Maerker T, Zimmermann U, Jurgens K, te Brinke H, Overlack N, Roepman R, Knipper M, Kremer H, Wolfrum U: Scaffold protein harmonin (USH1C) provides molecular links between Usher syndrome type 1 and type 2. Hum Mol Genet. 2005, 14: 3933-3943. 10.1093/hmg/ddi417.
van Wijk E, van der Zwaag B, Peters TA, Zimmermann U, te Brinke H, Kersten FFJ, Maerker T, Aller E, Hoefsloot LH, Cremers CWRJ, Cremers FPM, Wolfrum U, Knipper M, Roepman R, Kremer H: The DFNB31 gene product whirlin connects to the Usher protein network in the cochlea and retina by direct association with USH2A and VLGR1. Hum Mol Genet. 2006, 15: 751-765. 10.1093/hmg/ddi490.
Van Wijk E, Kersten FFJ, Kartono A, Mans DA, Brandwijk K, Letteboer SJF, Peters TA, Maerker T, Yan X, Cremers CWRJ, Cremers FPM, Wolfrum U, Roepman R, Kremer H: Usher syndrome and Leber congenital amaurosis are molecularly linked via a novel isoform of the centrosomal ninein-like protein. Hum Mol Genet. 2009, 18: 51-64.
Sedmak T, Wolfrum U: Intraflagellar transport molecules in ciliary and nonciliary cells of the retina. J Cell Biol. 2010, 189: 171-186. 10.1083/jcb.200911095.
Mack GJ, Compton DA: Analysis of mitotic microtubule-associated proteins using mass spectrometry identifies astrin, a spindle-associated protein. Proc Natl Acad Sci USA. 2001, 98: 14434-14439. 10.1073/pnas.261371298.
Thein KH, Kleylein-Sohn J, Nigg EA, Gruneberg U: Astrin is required for the maintenance of sister chromatid cohesion and centrosome integrity. J Cell Biol. 2007, 178: 345-354. 10.1083/jcb.200701163.
Cheng TS, Hsiao YL, Lin CC, Hsu CM, Chang MS, Lee CI, Yu RC, Huang CY, Howng SL, Hong YR: hNinein is required for targeting spindle-associated protein Astrin to the centrosome during the S and G2 phases. Exp Cell Res. 2007, 313: 1710-1721. 10.1016/j.yexcr.2007.02.023.
Wolfrum U, Salisbury JL: Expression of centrin isoforms in the mammalian retina. Exp Cell Res. 1998, 242: 10-17. 10.1006/excr.1998.4038.
Chang MS, Huang CJ, Chen ML, Chen ST, Fan CC, Chu JM, Lin WC, Yang YC: Cloning and characterization of hMAP126, a new member of mitotic spindle-associated proteins. Biochem Biophys Res Commun. 2001, 287: 116-121. 10.1006/bbrc.2001.5554.
Gruber J, Harborth J, Schnabel J, Weber K, Hatzfeld M: The mitotic-spindle-associated protein astrin is essential for progression through mitosis. J Cell Sci. 2002, 115: 4053-4059. 10.1242/jcs.00088.
Du J, Jablonski S, Yen TJ, Hannon GJ: Astrin regulates Aurora-A localization. Biochem Biophys Res Commun. 2008, 370: 213-219. 10.1016/j.bbrc.2008.03.072.
Casenghi M, Meraldi P, Weinhart U, Duncan PI, Korner R, Nigg EA: Polo-like kinase 1 regulates Nlp, a centrosome protein involved in microtubule nucleation. Dev Cell. 2003, 5: 113-125. 10.1016/S1534-5807(03)00193-X.
Casenghi M, Barr FA, Nigg EA: Phosphorylation of Nlp by Plk1 negatively regulates its dynein-dynactin-dependent targeting to the centrosome. J Cell Sci. 2005, 118: 5101-5108. 10.1242/jcs.02622.
Rapley J, Baxter JE, Blot J, Wattam SL, Casenghi M, Meraldi P, Nigg EA, Fry AM: Coordinate regulation of the mother centriole component nlp by nek2 and plk1 protein kinases. Mol Cell Biol. 2005, 25: 1309-1324. 10.1128/MCB.25.4.1309-1324.2005.
Yan J, Jin S, Li J, Zhan Q: Aurora B Interaction of Centrosomal Nlp Regulates Cytokinesis. J Biol Chem. 2010, 285: 40230-40239. 10.1074/jbc.M110.140541.
Yang YC, Hsu YT, Wu CC, Chen HT, Chang MS: Silencing of astrin induces the p53-dependent apoptosis by suppression of HPV18 E6 expression and sensitizes cells to paclitaxel treatment in HeLa cells. Biochem Biophys Res Commun. 2006, 343: 428-434. 10.1016/j.bbrc.2006.02.166.
Shao S, Liu R, Wang Y, Song Y, Zuo L, Xue L, Lu N, Hou N, Wang M, Yang X, Zhan Q: Centrosomal Nlp is an oncogenic protein that is gene-amplified in human tumors and causes spontaneous tumorigenesis in transgenic mice. J Clin Invest. 2010, 120: 498-507. 10.1172/JCI39447.
Wang Y, Zhan Q: Cell cycle-dependent expression of centrosomal ninein-like protein in human cells is regulated by the anaphase-promoting complex. J Biol Chem. 2007, 282: 17712-17719. 10.1074/jbc.M701350200.
Christensen ST, Pedersen LB, Schneider L, Satir P: Sensory cilia and integration of signal transduction in human health and disease. Traffic. 2007, 8: 97-109. 10.1111/j.1600-0854.2006.00516.x.
Singla V, Reiter JF: The primary cilium as the cell's antenna: signaling at a sensory organelle. Science. 2006, 313: 629-633. 10.1126/science.1124534.
Quarmby LM, Parker JD: Cilia and the cell cycle?. J Cell Biol. 2005, 169: 707-710. 10.1083/jcb.200503053.
Smith KR, Kieserman EK, Wang PI, Basten SG, Giles RH, Marcotte EM, Wallingford JB: A role for central spindle proteins in cilia structure and function. Cytoskeleton (Hoboken). 2011, 68: 112-124.
Liu Q, Tan G, Levenkova N, Li T, Pugh EN, Rux JJ, Speicher DW, Pierce EA: The proteome of the mouse photoreceptor sensory cilium complex. Mol Cell Proteomics. 2007, 6: 1299-1317. 10.1074/mcp.M700054-MCP200.
Roepman R, Wolfrum U: Protein networks and complexes in photoreceptor cilia. Subcell Biochem. 2007, 43: 209-235. 10.1007/978-1-4020-5943-8_10.
Wolfrum U, Schmitt A: Rhodopsin transport in the membrane of the connecting cilium of mammalian photoreceptor cells. Cell Motil Cytoskeleton. 2000, 46: 95-107. 10.1002/1097-0169(200006)46:2<95::AID-CM2>3.0.CO;2-Q.
Liu X, Bulgakov OV, Darrow KN, Pawlyk B, Adamian M, Liberman MC, Li T: Usherin is required for maintenance of retinal photoreceptors and normal development of cochlear hair cells. Proc Natl Acad Sci USA. 2007, 104: 4413-4418. 10.1073/pnas.0610950104.
Papermaster DS: The birth and death of photoreceptors: the Friedenwald Lecture. Invest Ophthalmol Vis Sci. 2002, 43: 1300-1309.
Famulski JK, Vos LJ, Rattner JB, Chan GK: Dynein/dynactin-mediated transport of kinetochore components off kinetochores and onto spindle poles induced by nordihydroguaiaretic acid. PLoS One. 2011, 6: e16494-10.1371/journal.pone.0016494.
Zallocchi M, Sisson JH, Cosgrove D: Biochemical characterization of native Usher protein complexes from a vesicular subfraction of tracheal epithelial cells. Biochemistry. 2010, 49: 1236-1247. 10.1021/bi9020617.
Xue J, Tarnasky HA, Rancourt DE, van der Hoorn FA: Targeted disruption of the testicular SPAG5/deepest protein does not affect spermatogenesis or fertility. Mol Cell Biol. 2002, 22: 1993-1997. 10.1128/MCB.22.7.1993-1997.2002.
Suzuki H, Yagi M, Suzuki K: Duplicated insertion mutation in the microtubule-associated protein Spag5 (astrin/MAP126) and defective proliferation of immature Sertoli cells in rat hypogonadic (hgn/hgn) testes. Reproduction. 2006, 132: 79-93. 10.1530/rep.1.01104.
Suzuki H, Yagi M, Saito K, Suzuki K: Embryonic pathogenesis of hypogonadism and renal hypoplasia in hgn/hgn rats characterized by male sterility, reduced female fertility and progressive renal insufficiency. Congenit Anom (Kyoto). 2007, 47: 34-44. 10.1111/j.1741-4520.2006.00138.x.
Suzuki H, Tokuriki T, Saito K, Hishida A, Suzuki K: Glomerular hyperfiltration and hypertrophy in the rat hypoplastic kidney as a model of oligomeganephronic disease. Nephrol Dial Transplant. 2005, 20: 1362-1369. 10.1093/ndt/gfh782.
Suzuki H, Tokuriki T, Kamita H, Oota C, Takasu M, Saito K, Suzuki K: Age-related pathophysiological changes in rat oligomeganephronic hypoplastic kidney. Pediatr Nephrol. 2006, 21: 637-642. 10.1007/s00467-006-0089-3.
Janin-Mercier A, Palcoux JB, Gubler MC, de Latour M, Dalens H, Fonck Y: Oligomeganephronic renal hypoplasia with tapetoretinal degeneration. Report of one case with ultrastructural study of the renal biopsy. Virchows Arch A Pathol Anat Histopathol. 1985, 407: 477-483. 10.1007/BF00709994.
Reiners J, Nagel-Wolfrum K, Jurgens K, Maerker T, Wolfrum U: Molecular basis of human Usher syndrome: deciphering the meshes of the Usher protein network provides insights into the pathomechanisms of the Usher disease. Exp Eye Res. 2006, 83: 97-119. 10.1016/j.exer.2005.11.010.
Roepman R, Schick D, Ferreira PA: Isolation of retinal proteins that interact with retinitis pigmentosa GTPase regulator by interaction trap screen in yeast. Methods Enzymol. 2000, 316: 688-704.
This research was supported by the Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands; The British Retinitis Pigmentosa Society (GR552, to RR and HK), the Heinsius Houbolt Foundation (to HK); The 'Landelijke Stichting voor Blinden en Slechtzienden' (RR and HK), the 'Stichting Blindenhulp' (to JK and HK); the Netherlands Organization for Scientific Research (NWO Vidi-91786396; to RR); and the European Community's Seventh Framework Programme FP7/2009 under grant agreement No 241955, SYSCILIA (to RR and HK).
The authors declare that they have no competing interests
FFJK carried out a major part of the experimental work, and was the primary author of the manuscript. EvW was closely involved during the whole process including some of the experiments, and contributed to writing the manuscript. LH carried out the real-time qPCR assays and was involved in a number of the other experiments, KB carried out the immunoelectron microscopy experiment, TAP was involved in the immunohistochemistry assays, MGA was involved in the protein-protein interaction experiments, and BvdZ performed a major part of the work for the in situ hybridization data. UW supervised the immunoelectron microscopy work and co-edited the manuscript. JEEK was co-supervisor of the PhD project, co-applicant of the major grant that supported this research, and critically read the manuscript. RR was co-supervisor of the study, co-applicant of grants that supported this research, and critically read the manuscript. HK was the primary supervisor of the project, co-applicant of the grants that supported this research, and contributed to the writing of this the manuscript. All authors read and approved the final manuscript.
These authors contributed equally to the work
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Kersten, F.F., van Wijk, E., Hetterschijt, L. et al. The mitotic spindle protein SPAG5/Astrin connects to the Usher protein network postmitotically. Cilia 1, 2 (2012). https://doi.org/10.1186/2046-2530-1-2
- Retinitis Pigmentosa
- Basal Body
- Photoreceptor Cell
- Ganglion Cell Layer
- Inner Nuclear Layer